Background: We aimed to investigate factors affecting ego-resilience, emotional labor, and job satisfaction and their relationship with Professional self-concept among psychiatric nurses. Methods: The study was conducted among nurses working in four psychiatric wards: Sung Eun Medical Foundation Reference Hospital (Medical Corporation) in Jeongeup City, Korea,Dasarang Hospital in Gwangju, Korea,Saemirae Hospital in Gwangju (metropolitan city), Korea,and Jangseong Hospital in Jangseong-eup City, Korea. All nurses fully understood the purpose of the study and voluntarily agreed to participate. From August 14 to September 24, 2020, direct survey data were collected by a researcher using a structured questionnaire. Of the 136 questionnaires retrieved, 129 were used for analysis, while 7 were excluded due to incomplete or missing responses. Results: Significantly different effects on Professional self-concept were noted for the following general characteristics: age (P<0. 001), religion (P=0. 024), duration of nursing career (P=0. 041), position (P=0. 021), salary (P=0. 016), nursing job satisfaction (P=0. 022), and psychiatric nursing job satisfaction (P=0. 010). Moreover, Professional self-concept was correlated with ego-resilience (r=0. 62, P<0. 001) and job satisfaction (r=0. 41, P<0. 001). In the multiple regression analysis, factors affecting Professional self-concept included no religious preference (β, =0. 25, P<0. 001), employment as a charge nurse (β, =-0. 20, P=0. 034), and ego-resilience (β, =0. 58, P<0. 001). The explanatory power was 42. 1%. Conclusion: Ego-resilience is the factor most strongly influencing Professional self-concept. Furthermore, Professional self-concept was stronger among those without religious preferences and those who held senior positions (charge nurse).